Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031802-100ug | 100ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Applications:
- Cell Culture
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Contactin 3;CNTN3
Accession:
NP_065923.1
Activity:
Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 104 cells/well are added to CNTN3 coated plates (0.8 ug/ml and 100 ul/well), approximately 35%-55% will adhere specifically after 60 minutes at 37°C.
Background:
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1; Contactin-2(TAG-1); Contactin-3(BIG-1); BIG-2; Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s; Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast; the function of the other four members of this subgroup remained unknown until recently. Contactin-3; also known as CNTN3 ( BIG-1 in rat and PANG in mouse ); is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells; amygdaloid and thalamic neurons and olfactory granule cells. In the brain; Contactin-3 is expressed in frontal lobe; occipital lobe; cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.
Calculated MW:
109.2 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
120-130 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ser1002; 708 Asp/Asn
Target Synonym:
BIG-1;PANG;PCS