Recombinant Mouse ICOS Ligand/ICOSL Protein (His & Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSM040816-100ug | 100ug | £615.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
ICOS Ligand;ICOSL
Accession:
NP_056605.1
Activity:
Immobilized human ICOS at 1 ug/ml (100 ul/well) can bind biotinylated mouse B7-H2 Fc chimera with a linear range of 0.125-1.0 ug/ml.
Background:
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.
Calculated MW:
54.3 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His-Fc
ObservedMW:
75-85 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Lys 279
Target Synonym:
Icosl;AU044799;B7-H2;B7RP-1;B7h;BG071784;GI50;GL50;GL50-B;ICOS-L;KIAA0653;LICOS;Ly115l;Mkiaa0653