Recombinant Rat Tie2/TEK Protein (Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSR030377-200ug | 200ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Tie2;TEK
Accession:
NP_001099207.1
Activity:
Immobilized S1h-3C-mANGPT2 at 10 ug/mL (100 uL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62ug/mL.
Background:
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Calculated MW:
108 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-hFc
ObservedMW:
120-130 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 4-Leu 743
Target Synonym:
Tie-2;Tie2