Daxx Antibody

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NSJ Bioreagents
Product Code: NSJ-V8935SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8935SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-DAXX-8C2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-8C2).
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FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8C2), and unstained cells (red).
3 / 4
IHC staining of FFPE human kidney tissue with Daxx antibody (clone PCRP-DAXX-8C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8C2) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-8C2).
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8C2), and unstained cells (red).
IHC staining of FFPE human kidney tissue with Daxx antibody (clone PCRP-DAXX-8C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8C2) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Daxx antibody should be determined by the researcher.
Description:
Daxx, or Death domain-associated protein 6, is a transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Death domain-associated protein 6 protein was used as the immunogen for the Daxx antibody.
Limitation:
This Daxx antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9UER7