ABO Antibody (Blood Group Antigen H Type 2)

Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

Optimal dilution of the Blood Group H2 antibody should be determined by the researcher.

This antibody reacts with H type 2 blood group epitope Fuca1-2 Galb1-4 GlcNAc. It does not cross-react with human blood group H type 1, 3 or 4, nor with the closely related type 2 antigen Ley and Lex. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular trans- formation. Blood group related antigens are usually mucin-type and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A (1 and 2), B, H (1 and 2), M, N, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. The expressions of the H1 and H2 in different cell types are con- trolled by different genes.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human breast cancer MCF-7 cells were used as the immunogen for the Blood Group H2 antibody. This antibody reacts with H type 2 blood group epitope Fuca1-2 Galb1-4 GlcNAc.

This Blood Group H2 antibody is available for research use only.

Cell Surface

Protein affinity

P16442