Negative Control Antibody for Mouse Monoclonals

NSJ Bioreagents
Product Code: NSJ-V8785SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8785SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: IGG1/453
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the Negative control antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human heart tissue with Negative control antibody (clone IGG1/453). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Neg

IHC staining of FFPE human heart tissue with Negative control antibody (clone IGG1/453). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free Neg

Documents

Further Information

Application Details:
ELISA; Flow cytometry; Immunofluorescence; Immunoprecipitation; Western blot; Immunohistochemistry: use at the same concentration as the primary antibody being controlled for
Application Note:
Optimal dilution of the Negative control antibody should be determined by the researcher.
Description:
To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human IG1R protein was used as the immunogen for the Negative control antibody.
Limitation:
This Negative control antibody is available for research use only.
Purity:
Protein A affinity