IRF9 Antibody / Interferon regulatory factor 9

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NSJ Bioreagents
Product Code: NSJ-V9381SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9381SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-IRF9-2F8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the IRF9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using IRF9 antibody (green, clone PCRP-IRF9-2F8) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with IRF9 antibody (blue, clone PCRP-IRF9-2F8), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free IRF9 antibody (clone PCRP-IRF9-2F8) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF9 antibody (clone PCRP-IRF9-2F8). These results demonstrate the foremost specificity of the PCRP-IRF9-2F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using IRF9 antibody (green, clone PCRP-IRF9-2F8) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with IRF9 antibody (blue, clone PCRP-IRF9-2F8), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free IRF9 antibody (clone PCRP-IRF9-2F8) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF9 antibody (clone PCRP-IRF9-2F8). These results demonstrate the foremost specificity of the PCRP-IRF9-2F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the IRF9 antibody should be determined by the researcher.
Description:
Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human IRF9 protein was used as the immunogen for the IRF9 antibody.
Limitation:
This IRF9 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q00978