CELF2 Antibody / CUGBP2 / ETR3

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NSJ Bioreagents
Product Code: NSJ-V9365SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9365SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-CELF2-1E4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the CELF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using CELF2 antibody (green) and phalloidin (red).
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Immunofluorescent staining of PFA-fixed human MCF-7 cells using CELF2 antibody (green) and phalloidin (red).
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Immunofluorescent staining of human U-87 cells using CELF2 antibody (green) and phalloidin (red).
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FACS staining of PFA-fixed human MCF7 cells with CELF2 antibody (blue) and unstained cells (red).
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FACS staining of PFA-fixed human HeLa cells with CELF2 antibody (blue) and unstained cells (red).
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FACS staining of PFA-fixed human U-87 cells with CELF2 antibody (blue) and unstained cells (red).
7 / 8
SDS-PAGE analysis of purified, BSA-free CELF2 antibody as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CELF2 antibody (clone PCRP-CELF2-1E4). These results demonstrate the foremost specificity of the PCRP-CELF2-1E4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using CELF2 antibody (green) and phalloidin (red).
Immunofluorescent staining of PFA-fixed human MCF-7 cells using CELF2 antibody (green) and phalloidin (red).
Immunofluorescent staining of human U-87 cells using CELF2 antibody (green) and phalloidin (red).
FACS staining of PFA-fixed human MCF7 cells with CELF2 antibody (blue) and unstained cells (red).
FACS staining of PFA-fixed human HeLa cells with CELF2 antibody (blue) and unstained cells (red).
FACS staining of PFA-fixed human U-87 cells with CELF2 antibody (blue) and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free CELF2 antibody as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CELF2 antibody (clone PCRP-CELF2-1E4). These results demonstrate the foremost specificity of the PCRP-CELF2-1E4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml)
Application Note:
Optimal dilution of the CELF2 antibody should be determined by the researcher.
Description:
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human CELF2/CUGBP2 protein was used as the immunogen for the CELF2 antibody.
Limitation:
This CELF2 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
O95319