FABP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9338SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9338SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: FABP1/3485
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FABP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human kidney tissue with FABP1 antibody (clone FABP1/3485). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Western blot analysis of human kidney tissue lysate using FABP1 antibody (clone FABP1/3485). Predicted molecular weight ~14 kDa.
3 / 4
SDS-PAGE analysis of purified, BSA-free FABP1 antibody (clone FABP1/3485) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FABP1 antibody (clone FABP1/3485). These results demonstrate the foremost specificity of the FABP1/3485 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human kidney tissue with FABP1 antibody (clone FABP1/3485). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human kidney tissue lysate using FABP1 antibody (clone FABP1/3485). Predicted molecular weight ~14 kDa.
SDS-PAGE analysis of purified, BSA-free FABP1 antibody (clone FABP1/3485) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FABP1 antibody (clone FABP1/3485). These results demonstrate the foremost specificity of the FABP1/3485 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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Further Information

Application Details:
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the FABP1 antibody should be determined by the researcher.
Description:
Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epithelium (E-FABP, psoriasis-associated FABP, PA-FABP), striated muscle and heart (H-FABP, mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP or FABP1), myelin (M-FABP) and testis (T-FABP). FABP1 (L-FABP) expression is modulated by developmental, hormonal, dietary and pharmacological factors, and is required for cholesterol synthesis and metabolism.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portin of amino acids 1-127 from the human protein was used as the immunogen for the FABP1 antibody.
Limitation:
This FABP1 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P07148