PDGF beta Antibody

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NSJ Bioreagents
Product Code: NSJ-V9327SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
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NSJ-V9327SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: PDGFB/3072
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the PDGFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PDGFB antibody (clone PDGFB/3072). These results demonstrate the foremost specificity of the PDGFB/3072 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC staining of FFPE human colon tissue with PDGFB antibody (clone PDGFB/3072). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free PDGFB antibody (clone PDGFB/3072) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PDGFB antibody (clone PDGFB/3072). These results demonstrate the foremost specificity of the PDGFB/3072 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human colon tissue with PDGFB antibody (clone PDGFB/3072). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free PDGFB antibody (clone PDGFB/3072) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the PDGFB antibody should be determined by the researcher.
Description:
PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms; PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 27-158 was used as the immunogen for the PDGFB antibody.
Limitation:
This PDGFB antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P01127