E-Cadherin Antibody / CDH1

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NSJ Bioreagents
Product Code: NSJ-V9299SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9299SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDH1/4585
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Cadherin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human kidney tissue with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cadherin 1 antibody (clone CDH1/4585). These results demonstrate the foremost specificity of the CDH1/4585 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free Cadherin 1 antibody (clone CDH1/4585) as confirmation of integrity and purity.
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IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cadherin 1 antibody (clone CDH1/4585). These results demonstrate the foremost specificity of the CDH1/4585 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Cadherin 1 antibody (clone CDH1/4585) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Cadherin 1 antibody should be determined by the researcher.
Description:
E-cadherin/Cadherin-1/CDH1 is a transmembrane, calcium dependent cell adhesion protein that mediates cell to cell adhesion and maintains structural and functional integrity of epithelial tissues.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Purified His-tagged CDH1/Cadherin 1 protein was used as the immunogen for the Cadherin 1 antibody.
Limitation:
This Cadherin 1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A/G affinity
Uniprot #:
P12830