SET Antibody / Protein SET / TAFI

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NSJ Bioreagents
Product Code: NSJ-V9261SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9261SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-SET-1C6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SET antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with SET antibody (blue, clone PCRP-SET-1C6) and isotype control (red).
2 / 4
Immunofluorescent staining of human HeLa cells using SET antibody (green, clone PCRP-SET-1C6) and phalloidin (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free SET antibody (clone PCRP-SET-1C6) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SET antibody (clone PCRP-SET-1C6). These results demonstrate the foremost specificity of the PCRP-SET-1C6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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FACS staining of PFA-fixed human HeLa cells with SET antibody (blue, clone PCRP-SET-1C6) and isotype control (red).
Immunofluorescent staining of human HeLa cells using SET antibody (green, clone PCRP-SET-1C6) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free SET antibody (clone PCRP-SET-1C6) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SET antibody (clone PCRP-SET-1C6). These results demonstrate the foremost specificity of the PCRP-SET-1C6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the SET antibody should be determined by the researcher.
Description:
Protein SET is a multitasking protein involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human SET protein was used as the immunogen for the SET antibody.
Limitation:
This SET antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q01105