PDX1 Antibody / Pancreas/duodenum homeobox protein 1

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NSJ Bioreagents
Product Code: NSJ-V9260SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9260SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-PDX1-2C11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the Pancreas/duodenum homeobox protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using Pancreas/duodenum homeobox protein 1 antibody (green, clone PCRP-PDX1-2C11) and phalloidin (red).
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FACS staining of PFA-fixed human HeLa cells with Pancreas/duodenum homeobox protein 1 antibody (blue, clone PCRP-PDX1-2C11), and unstained cells (red).
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SDS-PAGE analysis of purified, BSA-free Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11). These results demonstrate the foremost specificity of the PCRP-PDX1-2C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using Pancreas/duodenum homeobox protein 1 antibody (green, clone PCRP-PDX1-2C11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Pancreas/duodenum homeobox protein 1 antibody (blue, clone PCRP-PDX1-2C11), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11). These results demonstrate the foremost specificity of the PCRP-PDX1-2C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the Pancreas/duodenum homeobox protein 1 antibody should be determined by the researcher.
Description:
Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human PDX1 protein was used as the immunogen for the Pancreas/duodenum homeobox protein 1 antibody.
Limitation:
This Pancreas/duodenum homeobox protein 1 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P52945