RBM8A/Y14 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9259SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9259SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-RBM8A-1B4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the RBM8A/Y14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using RBM8A/Y14 antibody (green, clone PCRP-RBM8A-1B4) and phalloidin (red).
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FACS staining of PFA-fixed human HeLa cells with RBM8A/Y14 antibody (blue, clone PCRP-RBM8A-1B4), and unstained cells (red).
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBM8A/Y14 antibody (clone PCRP-RBM8A-1B4). These results demonstrate the foremost specificity of the PCRP-RBM8A-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using RBM8A/Y14 antibody (green, clone PCRP-RBM8A-1B4) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with RBM8A/Y14 antibody (blue, clone PCRP-RBM8A-1B4), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBM8A/Y14 antibody (clone PCRP-RBM8A-1B4). These results demonstrate the foremost specificity of the PCRP-RBM8A-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the RBM8A/Y14 antibody should be determined by the researcher.
Description:
The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human RBM8A protein was used as the immunogen for the RBM8A/Y14 antibody.
Limitation:
This RBM8A/Y14 antibody is available for research use only.
Localization:
Nucleus and cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9Y5S9