MSK1 Antibody / RPS6KA5

Contact us
NSJ Bioreagents
Product Code: NSJ-V9257SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9257SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-RPS6KA5-1A8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the RPS6KA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of human HeLa cells using RPS6KA5 antibody (green, clone PCRP-RPS6KA5-1A8) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with RPS6KA5 antibody (blue, clone PCRP-RPS6KA5-1A8) and isotype control (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RPS6KA5 antibody (clone PCRP-RPS6KA5-1A8). These results demonstrate the foremost specificity of the PCRP-RPS6KA5-1A8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free RPS6KA5 antibody (clone PCRP-RPS6KA5-1A8) as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of human HeLa cells using RPS6KA5 antibody (green, clone PCRP-RPS6KA5-1A8) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with RPS6KA5 antibody (blue, clone PCRP-RPS6KA5-1A8) and isotype control (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RPS6KA5 antibody (clone PCRP-RPS6KA5-1A8). These results demonstrate the foremost specificity of the PCRP-RPS6KA5-1A8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free RPS6KA5 antibody (clone PCRP-RPS6KA5-1A8) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the RPS6KA5 antibody should be determined by the researcher.
Description:
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human RPS6KA5 protein was used as the immunogen for the RPS6KA5 antibody.
Limitation:
This RPS6KA5 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
O75582