ZMYM3 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V9256SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9256SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZMYM3-2F10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ZMYM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using ZMYM3 antibody (green, clone PCRP-ZMYM3-2F10) and phalloidin (red).
2 / 5
IHC staining of FFPE human breast carcinoma tissue with ZMYM3 antibody (clone PCRP-ZMYM3-2F10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
FACS staining of PFA-fixed human HeLa cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).
4 / 5
FACS staining of PFA-fixed human Raji cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZMYM3 antibody (clone PCRP-ZMYM3-2F10). These results demonstrate the foremost specificity of the PCRP-ZMYM3-2F10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using ZMYM3 antibody (green, clone PCRP-ZMYM3-2F10) and phalloidin (red).
IHC staining of FFPE human breast carcinoma tissue with ZMYM3 antibody (clone PCRP-ZMYM3-2F10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).
FACS staining of PFA-fixed human Raji cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZMYM3 antibody (clone PCRP-ZMYM3-2F10). These results demonstrate the foremost specificity of the PCRP-ZMYM3-2F10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the ZMYM3 antibody should be determined by the researcher.
Description:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZMYM3 (zinc finger MYM-type protein 3), also known as ZNF261 (zinc finger protein 261), XFIM, DXS6673E or MYM, is a 1,370 amino acid nuclear protein that contains nine MYM-type zinc fingers. Expressed in a variety of tissues, including heart, muscle and brain, ZMYM3 is thought to function as part of a histone deacetylase-containing complex that contains other proteins, such as HDAC1 and HDAC2, and may play a role in gene silencing through the modification of chromatin structure. Defects in the gene encoding ZMYM3 that lead to chromosomal translocations may be a cause of X-linked mental retardation. Two isoforms of ZMYM3 exist due to alternative splicing events.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ZMYM3 protein was used as the immunogen for the ZMYM3 antibody.
Limitation:
This ZMYM3 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q14202