ZNF622 Antibody / ZPR9

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NSJ Bioreagents
Product Code: NSJ-V9254SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9254SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZNF622-1C11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the ZNF622 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF622 antibody (green, clone PCRP-ZNF622-1C11) and phalloidin (red).
2 / 3
FACS staining of PFA-fixed human HeLa cells with ZNF622 antibody (blue, clone PCRP-ZNF622-1C11), and unstained cells (red).
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF622 antibody (clone PCRP-ZNF622-1C11). These results demonstrate the foremost specificity of the PCRP-ZNF622-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF622 antibody (green, clone PCRP-ZNF622-1C11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ZNF622 antibody (blue, clone PCRP-ZNF622-1C11), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF622 antibody (clone PCRP-ZNF622-1C11). These results demonstrate the foremost specificity of the PCRP-ZNF622-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the ZNF622 antibody should be determined by the researcher.
Description:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF622 (zinc-finger protein 622), also known as ZPR9 (zinc-fingerlike protein 9), is a 477 amino acid protein that localizes to both the nucleus and the cytoplasm. Expressed in liver, spleen, lung, kidney and brain, ZNF622 is thought to activate the bound transcription factor B-Myb and, through this activation, may play a role in embryonic development. ZNF622 contains two U1-type zinc fingers and exists as either a homodimer or a heterodimer that can be phosphorylated by MELK (maternal embryonic leucine zipper kinase). Overexpression of ZNF266 may be associated with liver metastases, carcinomatoses and colorectal carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ZNF622 protein was used as the immunogen for the ZNF622 antibody.
Limitation:
This ZNF622 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q969S3