TSC22D1 Antibody / TSC22

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NSJ Bioreagents
Product Code: NSJ-V9253SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9253SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-TSC22D1-1A2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the TSC22D1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using TSC22D1 antibody (green, clone PCRP-TSC22D1-1A2) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with TSC22D1 antibody (blue, clone PCRP-TSC22D1-1A2), and unstained cells (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TSC22D1 antibody (clone PCRP-TSC22D1-1A2). These results demonstrate the foremost specificity of the PCRP-TSC22D1-1A2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free TSC22D1 antibody (PCRP-TSC22D1-1A2) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells using TSC22D1 antibody (green, clone PCRP-TSC22D1-1A2) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with TSC22D1 antibody (blue, clone PCRP-TSC22D1-1A2), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TSC22D1 antibody (clone PCRP-TSC22D1-1A2). These results demonstrate the foremost specificity of the PCRP-TSC22D1-1A2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free TSC22D1 antibody (PCRP-TSC22D1-1A2) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the TSC22D1 antibody should be determined by the researcher.
Description:
TSC22 domain family protein 1 is a transcriptional repressor which acts on the C-type natriuretic peptide (CNP) promoter. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human TSC22D1 protein was used as the immunogen for the TSC22D1 antibody.
Limitation:
This TSC22D1 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q15714