TIF1 alpha Antibody / TIF1A / TRIM24

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NSJ Bioreagents
Product Code: NSJ-V9252SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9252SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-TRIM24-1B12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the TIF1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed MCF7 cells using TIF1 alpha antibody (green, clone PCRP-TRIM24-1B12) and phalloidin (red).
2 / 3
FACS staining of PFA-fixed human HeLa cells with TIF1 alpha antibody (blue, clone PCRP-TRIM24-1B12) and isotype control (red).
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TIF1 alpha antibody (clone PCRP-TRIM24-1B12). These results demonstrate the foremost specificity of the PCRP-TRIM24-1B12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed MCF7 cells using TIF1 alpha antibody (green, clone PCRP-TRIM24-1B12) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with TIF1 alpha antibody (blue, clone PCRP-TRIM24-1B12) and isotype control (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TIF1 alpha antibody (clone PCRP-TRIM24-1B12). These results demonstrate the foremost specificity of the PCRP-TRIM24-1B12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the TIF1 alpha antibody should be determined by the researcher.
Description:
TIF1 alpha, also referred to as TIF1A and TRIM24, is a transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Transcription intermediary factor 1-alpha protein was used as the immunogen for the TIF1 alpha antibody.
Limitation:
This TIF1 alpha antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
O15164