SCXA Antibody / Scleraxis

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NSJ Bioreagents
Product Code: NSJ-V9241SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9241SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-SCXA-2D11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the SCXA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of human HeLa cells using SCXA antibody (green, clone PCRP-SCXA-2D11) and phalloidin (red).
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FACS staining of PFA-fixed human HeLa cells with SCXA antibody (blue, clone PCRP-SCXA-2D11), and unstained cells (red).
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SCXA antibody (clone PCRP-SCXA-2D11). These results demonstrate the foremost specificity of the PCRP-SCXA-2D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of human HeLa cells using SCXA antibody (green, clone PCRP-SCXA-2D11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with SCXA antibody (blue, clone PCRP-SCXA-2D11), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SCXA antibody (clone PCRP-SCXA-2D11). These results demonstrate the foremost specificity of the PCRP-SCXA-2D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the SCXA antibody should be determined by the researcher.
Description:
Transcription factors are proteins that bind DNA adjacent to genes and control the production of mRNA transcripts. Scleraxis (basic helix-loop-helix transcription factor scleraxis) is a 201 amino acid protein that dimerizes with another bHLH protein to initiate transcription. Scleraxis is known to play a role in formation of mesoderm and somite-derived chondrogenic lineages. Scleraxis localizes to the nucleus and contains one bHLH domain. bHLH transcription factors, in general, function in cellular differentiation, proliferation, and oncogene regulation. The gene encoding Scleraxis maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Basic helix-loop-helix transcription factor scleraxis protein was used as the immunogen for the SCXA antibody.
Limitation:
This SCXA antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q7RTU7