POLE3 Antibody / CHRAC-17
Code | Size | Price |
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NSJ-V9234SAF-100UG | 100ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-POLE3-2F10
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the POLE3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Further Information
Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the POLE3 antibody should be determined by the researcher.
Description:
DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human DNA polymerase epsilon subunit 3 protein was used as the immunogen for the POLE3 antibody.
Limitation:
This POLE3 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9NRF9