NMI Antibody / N-myc and STAT interactor / N-Myc Interactor

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NSJ Bioreagents
Product Code: NSJ-V9219SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9219SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-NMI-1C1
Regulatory Status: RUO
Target Species: Human
Application: Fluorescence-activated cell sorting (FACS)
Storage:
Aliquot the N-Myc Interactor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using N-Myc Interactor antibody (clone PCRP-NMI-1C1). These results demonstrate the foremost specificity of the PCRP-NMI-1C1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
FACS staining of PFA-fixed human HeLa cells with N-Myc Interactor antibody (blue, clone PCRP-NMI-1C1), and unstained cells (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free N-Myc Interactor antibody (clone PCRP-NMI-1C1) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using N-Myc Interactor antibody (clone PCRP-NMI-1C1). These results demonstrate the foremost specificity of the PCRP-NMI-1C1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with N-Myc Interactor antibody (blue, clone PCRP-NMI-1C1), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free N-Myc Interactor antibody (clone PCRP-NMI-1C1) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the N-Myc Interactor antibody should be determined by the researcher.
Description:
Nmi (for N-Myc interactor) is an interferon inducible protein that associates with multiple transcription factors, including c-Myc, n-Myc, Max, and c-Fos, which contain bHLH-ZIP, bHLH, or Zip domains. Nmi is ubiquitously expressed at low levels throughout various fetal and adult tissues and at higher levels in myeloid leukemias and cell lines overexpressing c-Myc. In addition to binding Myc proteins, Nmi also associates with the Stat family of transcription factors, where it enhances Stat-dependent transcription. Although Nmi lacks an intrinsic DNA binding or activation domain, Nmi enhances the transcriptional activity of the Stat proteins, in response to cytokine stimulation, by recruiting the Stat1 and Stat5 transcriptional coactivators, CREB-binding protein (CBP) and p300. In vitro studies indicate that Nmi, expressed in conjunction with CBP, enhances the transcriptional responsiveness of Stat5 to IL-2 stimulation five-fold over CBP alone by increasing the affinity of Stat proteins for CBP/p300.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human N-Myc Interactor protein was used as the immunogen for the N-Myc Interactor antibody.
Limitation:
This N-Myc Interactor antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q13287