CD2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9217SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9217SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: LFA2/7102
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the CD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human lymph node tissue with CD2 antibody (clone LFA2/7102) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with C2 antibody (clone LFA2/7102) at 2ug/ml in PBS for 30min RT. Strong cell surface staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human tonsil tissue with CD2 antibody (clone LFA2/7102). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone LFA2/7102) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD2 antibody (clone LFA2/7102). These results demonstrate the foremost specificity of the LFA2/7102 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human lymph node tissue with CD2 antibody (clone LFA2/7102) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with C2 antibody (clone LFA2/7102) at 2ug/ml in PBS for 30min RT. Strong cell surface staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD2 antibody (clone LFA2/7102). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone LFA2/7102) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD2 antibody (clone LFA2/7102). These results demonstrate the foremost specificity of the LFA2/7102 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CD2 antibody should be determined by the researcher.
Description:
CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed. It can be used to label the diagnosis and study of normal T cells and their associated tumors, such as pre-t cell lymphoma, marginal T cell lymphoma and anaplastic cell lymphoma. CD2 antibody is useful for identification of precursor and mature T-cell lymphomas. Aberrant loss of CD2 in T-cell lymphomas may help to distinguish them from reactive T-cell proliferations.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein fragment was used as the immunogen for the CD2 antibody.
Limitation:
This CD2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A/G affinity
Uniprot #:
P06729