E4F1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9189SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9189SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-E4F1-2D1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the E4F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with E4F1 antibody (blue, clone PCRP-E4F1-2D1), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free E4F1 antibody (clone PCRP-E4F1-2D1) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using E4F1 antibody (clone PCRP-E4F1-2D1). These results demonstrate the foremost specificity of the PCRP-E4F1-2D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with E4F1 antibody (blue, clone PCRP-E4F1-2D1), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free E4F1 antibody (clone PCRP-E4F1-2D1) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using E4F1 antibody (clone PCRP-E4F1-2D1). These results demonstrate the foremost specificity of the PCRP-E4F1-2D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the E4F1 antibody should be determined by the researcher.
Description:
E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human E4F1 protein was used as the immunogen for the E4F1 antibody.
Limitation:
This E4F1 antibody is available for research use only.
Localization:
Nucleus, cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q66K89