Daxx Antibody

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NSJ Bioreagents
Product Code: NSJ-V9187SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9187SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-DAXX-8B7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-8B7). These results demonstrate the foremost specificity of the PCRP-DAXX-8B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
Immunofluorescent staining of human U87 cells using Daxx antibody (green, clone PCRP-DAXX-8B7) and phalloidin (red).
3 / 4
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8B7), and unstained cells (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8B7) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-8B7). These results demonstrate the foremost specificity of the PCRP-DAXX-8B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of human U87 cells using Daxx antibody (green, clone PCRP-DAXX-8B7) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8B7), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8B7) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the Daxx antibody should be determined by the researcher.
Description:
Daxx interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, it functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.
Limitation:
This Daxx antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9UER7