CD38 Antibody

NSJ Bioreagents
Product Code: NSJ-V9174SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9174SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CD38/4328
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD38 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human 1) spleen and 2) Raji cell lysate using CD38 antibody (clone CD384328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
2 / 6
Western blot testing of human Raji cell lysate using CD38 antibody (clone CD38/4328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
3 / 6
IHC staining of FFPE human tonsil tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human prostate carcinoma tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
Negative control: IHC staining of FFPE human cerebral cortex tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD38 antibody (clone CD38/4328). These results demonstrate the foremost specificity of the CD38/4328 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human 1) spleen and 2) Raji cell lysate using CD38 antibody (clone CD384328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
Western blot testing of human Raji cell lysate using CD38 antibody (clone CD38/4328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
IHC staining of FFPE human tonsil tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human cerebral cortex tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD38 antibody (clone CD38/4328). These results demonstrate the foremost specificity of the CD38/4328 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/million cells,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CD38 antibody should be determined by the researcher.
Description:
CD38 is a type II transmembrane glycoprotein that is present on early B- and T-cell lineages and activated B- and T-cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B-cells, mitogen-activated T-cells, monocytes and Ig-secreting plasma cells. CD38 is expressed on CD34+ cells. The CD34+CD38- population of hematopoietic stems cells define the most pluripotent cells (e.g. blast colony forming cells).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human CD38 protein was used as the immunogen for the CD38 antibody.
Limitation:
This CD38 antibody is available for research use only.
Localization:
Cell surface and cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P28907