APOD Antibody / Apolipoprotein D

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NSJ Bioreagents
Product Code: NSJ-V9150SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9150SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: APOD/3415
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the APOD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human liver tissue with Apolipoprotein D antibody (clone APOD/3415). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
SDS-PAGE analysis of purified, BSA-free APOD antibody (clone APOD/3415) as confirmation of integrity and purity.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APOD antibody (clone APOD/3415). These results demonstrate the foremost specificity of the APOD/3415 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human liver tissue with Apolipoprotein D antibody (clone APOD/3415). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free APOD antibody (clone APOD/3415) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APOD antibody (clone APOD/3415). These results demonstrate the foremost specificity of the APOD/3415 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the APOD antibody should be determined by the researcher.
Description:
Lipids, such as phospholipids, triacylglycerols and cholesterol, are weakly soluble in aqueous solution and therefore are transported by circulation as components of lipoproteins. Lipoproteins are globular particles that consist of a non-polar core of triacylglycerols and cholesteryl esters surrounded by phospholipid, cholesterol and an amphiphilic coating of protein, known as apolipoproteins (apo). These complexes allow the dissolution and shuttling of their non-polar lipid components. At least nine different apolipoproteins are distributed in significant amounts in different human lipoproteins. Apolipoprotein D (apoD) is a member of the lipocalin superfamily of transporter proteins that bind small hydrophobic molecules, including arachidonic acid (AA). The ability of apoD to bind AA implicates it in pathways associated with membrane phospholipid signal transduction and metabolism. apoD expression has been shown to correlate both with cell cycle arrest and with prognosis in several types of malignancy, including central nervous system astrocytomas and medulloblastomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portin of amino acids 21-119 from the human protein was used as the immunogen for the APOD antibody.
Limitation:
This APOD antibody is available for research use only.
Localization:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
P05090