RBMS2 Antibody / SCR3

Contact us
NSJ Bioreagents
Product Code: NSJ-V8997SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8997SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-RBMS2-1B6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunoprecipitation (IP)
Storage:
Aliquot the RBMS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
FACS staining of PFA-fixed human HeLa cells with RBMS2 antibody (blue, clone PCRP-RBMS2-1B6) and isotype control (red).
2 / 3
SDS-PAGE analysis of purified, BSA-free RBMS2 antibody (PCRP-RBMS2-1B6) as confirmation of integrity and purity.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBMS2 antibody (clone PCRP-RBMS2-1B6). These results demonstrate the foremost specificity of the PCRP-RBMS2-1B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

FACS staining of PFA-fixed human HeLa cells with RBMS2 antibody (blue, clone PCRP-RBMS2-1B6) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free RBMS2 antibody (PCRP-RBMS2-1B6) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RBMS2 antibody (clone PCRP-RBMS2-1B6). These results demonstrate the foremost specificity of the PCRP-RBMS2-1B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
ELISA (For coating, order BSA-free format),Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the RBMS2 antibody should be determined by the researcher.
Description:
RBMS2 is a member of a small family of proteins that bind single stranded DNA or RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, and are required for DNA binding. The RBMS proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. RBMS2 (RNA-binding motif, single-stranded-interacting protein 2) is a 407 amino acid protein that contains 2 RRM (RNA recognition motif) domains and localizes to nucleus. It has been suggested that RBMS2 suppresses Cdc2 kinase and Cdc13 cyclin mutants through the induction of translation of Cdc2. The RBMS2 gene is conserved in chimpanzee, canine, bovine, mouse, rat and zebrafish, and maps to human chromosome 12q13.3.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human RBMS2 protein was used as the immunogen for the RBMS2 antibody.
Limitation:
This RBMS2 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q15434