SP100 Antibody
Code | Size | Price |
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NSJ-V8994SAF-100UG | 100ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-SP100-1B9
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Aliquot the SP100 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Images
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Further Information
Application Details:
ELISA (For coating, order BSA-free format),Western blot: 1-2ug/ml,Immunofluorescence: 0.5-2ug/ml,Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 0.5-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the SP100 antibody should be determined by the researcher.
Description:
The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human SP100 protein was used as the immunogen for the SP100 antibody.
Limitation:
This SP100 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P23497