FRA2 Antibody / FOSL2

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NSJ Bioreagents
Product Code: NSJ-V8958SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8958SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-FOSL2-1B1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the FRA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with FRA2 antibody (blue, clone PCRP-FOSL2-1B1), and isotype control (red).
2 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using FRA2 antibody (green, clone PCRP-FOSL2-1B1) and phalloidin (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free FRA2 antibody (PCRP-FOSL2-1B1) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FRA2 antibody (clone PCRP-FOSL2-1B1). These results demonstrate the foremost specificity of the PCRP-FOSL2-1B1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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FACS staining of PFA-fixed human HeLa cells with FRA2 antibody (blue, clone PCRP-FOSL2-1B1), and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using FRA2 antibody (green, clone PCRP-FOSL2-1B1) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free FRA2 antibody (PCRP-FOSL2-1B1) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FRA2 antibody (clone PCRP-FOSL2-1B1). These results demonstrate the foremost specificity of the PCRP-FOSL2-1B1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the FRA2 antibody should be determined by the researcher.
Description:
The Fos related gene, Fra-2, was initially molecularly cloned from chicken genomic DNA and shown to represent a new member of the immediate early gene family. The human counterpart of the chicken Fra-2 gene has since been described. Sequence alignment shows that the amino acid sequences conserved among Fra-2, c-Fos, Fra-1 and Fos B are contained in five regions. Region 2, the longest and most highly conserved region, contains the leucine zipper structure and the basic region, suggesting that like Fos, Fra-1 and Fos B, Fra-2 also forms heterodimers with c-Jun that recognize a specific DNA sequence such as the binding site for transcription factor AP-1. Such a model is further supported by the finding that the Fra-2 gene product forms a complex with c-Jun in growth-stimulated cells
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human FOSL2 protein was used as the immunogen for the FRA2 antibody.
Limitation:
This FRA2 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P15408