APOB Antibody / Apolipoprotein B

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NSJ Bioreagents
Product Code: NSJ-V8941SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8941SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: APOB/4332
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the APOB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human liver tissue with APOB antibody (clone APOB/4332). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
SDS-PAGE analysis of purified, BSA-free APOB antibody (clone APOB/4332) as confirmation of integrity and purity.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APOB antibody (clone APOB/4332). These results demonstrate the foremost specificity of the APOB/4332 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human liver tissue with APOB antibody (clone APOB/4332). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free APOB antibody (clone APOB/4332) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APOB antibody (clone APOB/4332). These results demonstrate the foremost specificity of the APOB/4332 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the APOB antibody should be determined by the researcher.
Description:
Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. ApoB-100 is a protein primarily synthesized in the liver as a structural component of very low density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portin of amino acids 592-689 from the human protein was used as the immunogen for the APOB antibody.
Limitation:
This APOB antibody is available for research use only.
Localization:
Cytoplasm, Secreted
Purity:
Protein A/G affinity
Uniprot #:
P04114