ID1 Antibody / DNA binding protein inhibitor ID-1

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NSJ Bioreagents
Product Code: NSJ-V8940SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8940SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ID1-2F11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the ID1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using ID1 antibody (green, clone PCRP-ID1-2F11) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with ID1 antibody (blue, clone PCRP-ID1-2F11) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free ID1 antibody (clone PCRP-ID1-2F11) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ID1 antibody (clone PCRP-ID1-2F11). These results demonstrate the foremost specificity of the PCRP-ID1-2F11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using ID1 antibody (green, clone PCRP-ID1-2F11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ID1 antibody (blue, clone PCRP-ID1-2F11) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free ID1 antibody (clone PCRP-ID1-2F11) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ID1 antibody (clone PCRP-ID1-2F11). These results demonstrate the foremost specificity of the PCRP-ID1-2F11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the ID1 antibody should be determined by the researcher.
Description:
Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 5-150 was used as the immunogen for the ID1 antibody.
Limitation:
This ID1 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P41134