C-ets-2 Antibody / ETS2

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NSJ Bioreagents
Product Code: NSJ-V8921SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8921SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ETS2-1D9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the ETS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ETS2 antibody (clone PCRP-ETS2-1D9). These results demonstrate the foremost specificity of the PCRP-ETS2-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells susing ETS2 antibody (green, clone PCRP-ETS2-1D9) and phalloidin (red).
3 / 4
FACS staining of PFA-fixed human HeLa cells with ETS2 antibody (blue, clone PCRP-ETS2-1D9) and isotype control (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free ETS2 antibody (clone PCRP-ETS2-1D9) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ETS2 antibody (clone PCRP-ETS2-1D9). These results demonstrate the foremost specificity of the PCRP-ETS2-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of PFA-fixed human HeLa cells susing ETS2 antibody (green, clone PCRP-ETS2-1D9) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ETS2 antibody (blue, clone PCRP-ETS2-1D9) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free ETS2 antibody (clone PCRP-ETS2-1D9) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the ETS2 antibody should be determined by the researcher.
Description:
ETS2 (Protein C-ets-2) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. ETS2 was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. ETS2 protein expression is complex and changes throughout development. ETS2 appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ETS2 protein was used as the immunogen for the ETS2 antibody.
Limitation:
This ETS2 antibody is available for research use only.
Localization:
Nucleus, cytoplasm, plasma membrane
Purity:
Protein A/G affinity
Uniprot #:
P15036