PBX1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8884SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8884SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-PBX1-3C8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the PBX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with PBX1 antibody (blue, clone PCRP-PBX1-3C8) and isotype control (red).
2 / 4
SDS-PAGE analysis of purified, BSA-free PBX1 antibody (PCRP-PBX1-3C8) as confirmation of integrity and purity.
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using PBX1 antibody (green, clone PCRP-PBX1-3C8) and phalloidin (red).
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PBX1 antibody (clone PCRP-PBX1-3C8). These results demonstrate the foremost specificity of the PCRP-PBX1-3C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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FACS staining of PFA-fixed human HeLa cells with PBX1 antibody (blue, clone PCRP-PBX1-3C8) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free PBX1 antibody (PCRP-PBX1-3C8) as confirmation of integrity and purity.
Immunofluorescent staining of PFA-fixed human HeLa cells using PBX1 antibody (green, clone PCRP-PBX1-3C8) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PBX1 antibody (clone PCRP-PBX1-3C8). These results demonstrate the foremost specificity of the PCRP-PBX1-3C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the PBX1 antibody should be determined by the researcher.
Description:
Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human PBX1 protein was used as the immunogen for the PBX1 antibody.
Limitation:
This PBX1 antibody is available for research use only.
Localization:
Nucleus, cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P40424