ZHX3 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8872SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8872SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZHX3-1D11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the ZHX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZHX3 antibody (clone PCRP-ZHX3-1D11). These results demonstrate the foremost specificity of the PCRP-ZHX3-1D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
FACS staining of PFA-fixed human HeLa cells with ZHX3 antibody (blue, clone PCRP-ZHX3-1D11) and isotype control (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free ZHX3 antibody (clone PCRP-ZHX3-1D11) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZHX3 antibody (clone PCRP-ZHX3-1D11). These results demonstrate the foremost specificity of the PCRP-ZHX3-1D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with ZHX3 antibody (blue, clone PCRP-ZHX3-1D11) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free ZHX3 antibody (clone PCRP-ZHX3-1D11) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the ZHX3 antibody should be determined by the researcher.
Description:
Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ZHX3 protein was used as the immunogen for the ZHX3 antibody.
Limitation:
This ZHX3 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q9H4I2