IGF1R Antibody / Insulin like growth factor 1 receptor

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NSJ Bioreagents
Product Code: NSJ-V8864SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8864SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: IGF1R/4667
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the IGF1R antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human prostate carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human breast carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human colon carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free IGF1R antibody (clone IGF1R/4667) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IGF1R antibody (clone IGF1R/4667). These results demonstrate the foremost specificity of the IGF1R/4667 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human prostate carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free IGF1R antibody (clone IGF1R/4667) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IGF1R antibody (clone IGF1R/4667). These results demonstrate the foremost specificity of the IGF1R/4667 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the IGF1R antibody should be determined by the researcher.
Description:
Receptor tyrosine kinases (RTKs) are transmembrane molecular scaffolds that influence cellular processes including the cell cycle, cell migration, cell metabolism, cell survival, proliferation and differentiation. Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand Insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of Insulin receptor substrate (IRS)-1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals. The human IGF-IR gene maps to chromosome 15q26.3 and encodes a 1,376 amino acid precursor protein that cleaves into subunits. The human IGF-IIR gene maps to chromosome 6q26 and encodes a 2,491 amino acid transmembrane protein.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 550-750 was used as the immunogen for the IGF1R antibody.
Limitation:
This IGF1R antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
P08069