MITF Antibody

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NSJ Bioreagents
Product Code: NSJ-V8800SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8800SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-MITF-1D9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with MITF antibody (blue, clone PCRP-MITF-1D9) and isotype control (red).
2 / 4
SDS-PAGE analysis of purified, BSA-free MITF antibody (PCRP-MITF-1D9) as confirmation of integrity and purity.
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using MITF antibody (green, clone PCRP-MITF-1D9) and phalloidin (red).
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MITF antibody (clone PCRP-MITF-1D9). These results demonstrate the foremost specificity of the PCRP-MITF-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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FACS staining of PFA-fixed human HeLa cells with MITF antibody (blue, clone PCRP-MITF-1D9) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free MITF antibody (PCRP-MITF-1D9) as confirmation of integrity and purity.
Immunofluorescent staining of PFA-fixed human HeLa cells using MITF antibody (green, clone PCRP-MITF-1D9) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MITF antibody (clone PCRP-MITF-1D9). These results demonstrate the foremost specificity of the PCRP-MITF-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the MITF antibody should be determined by the researcher.
Description:
MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human MITF protein was used as the immunogen for the MITF antibody.
Limitation:
This MITF antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
O75030