MIF Antibody / Macrophage migration inhibitory factor

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NSJ Bioreagents
Product Code: NSJ-V8799SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8799SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: MIF/3488
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Macrophage migration inhibitory factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) LNCaP and 2) PC3 cell lysates using Macrophage migration inhibitory factor antibody (clone MIF/3488). Predicted molecular weight ~13 kDa.
2 / 4
IHC staining of FFPE human prostate tissue with Macrophage migration inhibitory factor antibody (clone MIF/3488). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free Macrophage migration inhibitory factor antibody (MIF/3488) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Macrophage migration inhibitory factor antibody (clone MIF/3488). These results demonstrate the foremost specificity of the MIF/3488 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human 1) LNCaP and 2) PC3 cell lysates using Macrophage migration inhibitory factor antibody (clone MIF/3488). Predicted molecular weight ~13 kDa.
IHC staining of FFPE human prostate tissue with Macrophage migration inhibitory factor antibody (clone MIF/3488). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Macrophage migration inhibitory factor antibody (MIF/3488) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Macrophage migration inhibitory factor antibody (clone MIF/3488). These results demonstrate the foremost specificity of the MIF/3488 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Macrophage migration inhibitory factor antibody should be determined by the researcher.
Description:
Macrophage migration inhibitory factor, known as MIF or glycosylation inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein fragment was used as the immunogen for the Macrophage migration inhibitory factor antibody.
Limitation:
This Macrophage migration inhibitory factor antibody is available for research use only.
Localization:
Secreted, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P14174