IL15 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8776SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8776SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: IL15/4354
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the IL15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IL15 antibody (clone IL15/4354). These results demonstrate the foremost specificity of the IL15/4354 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC staining of FFPE human adrenal gland tissue with IL15 antibody (clone IL15/4354). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free IL15 antibody (IL15/4354) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IL15 antibody (clone IL15/4354). These results demonstrate the foremost specificity of the IL15/4354 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human adrenal gland tissue with IL15 antibody (clone IL15/4354). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free IL15 antibody (IL15/4354) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the IL15 antibody should be determined by the researcher.
Description:
Interleukin-15 (IL-15), also designated IL-T, is a cloned cytokine which shares several biological activities but no sequence homology with IL-2. Human, mouse and simian IL-15 cDNA clones have been isolated and characterized. All 3 species encode a 162 amino acid residue precursor protein containing a 48 amino acid leader that is cleaved to generate the mature form of IL-15. IL-15 stimulates the proliferation of T cells and NK cells, while enhancing B cell expansion and antibody production. Unlike IL-2, IL-15 is not produced by lymphocytes, but appears to be produced by macrophages, epithelial lines, muscle and placenta. IL-15 has also been shown to be a chemoattractant for human blood T lymphocytes and to be able to induce lymphokine-activated killer (LAK) activity in NK cells as well as to be able to induce the generation of cytolytic effector cells. Studies have shown that IL-15 is the only other cytokine that shares the subunit.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein fragment was used as the immunogen for the IL15 antibody.
Limitation:
This IL15 antibody is available for research use only.
Localization:
Secreted, Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
IDP40933