p40 / deltaNp63 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9359SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9359SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: P40/4396R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant deltaNp63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
FACS staining of PFA-fixed human HeLa cells with recombinant deltaNp63 antibody (blue, clone P40/4396R) and isotype control (red).
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IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with recombinant deltaNp63 antibody (blue, clone P40/4396R) and isotype control (red).

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant deltaNp63 antibody should be determined by the researcher.
Description:
p40, the deltaN form of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-100 was used as the immunogen for the recombinant deltaNp63 antibody.
Limitation:
This recombinant deltaNp63 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A affinity
Uniprot #:
Q9H3D4