CD5 Antibody

Immunohistochemistry (FFPE): 1-2ug/ml

Optimal dilution of the recombinant CD5 antibody should be determined by the researcher.

The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A portion of amino acids 450-495 from the intracellular region of human CD5 was used as the immunogen for the recombinant CD5 antibody.

This recombinant CD5 antibody is available for research use only.

Cell Surface

Protein A/G affinity

P06127