MPO Antibody / Myeloperoxidase

NSJ Bioreagents
Product Code: NSJ-V9329SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9329SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rMPO/6904
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant MPO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human bone marrow with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human spleen tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human spleen tissue lysate using recombinant MPO antibody (clone rMPO/6904). Expected molecular weight: 75-90 kDa (pro form).
5 / 5
SDS-PAGE analysis of purified, BSA-free MPO antibody (clone rMPO/6904) as confirmation of integrity and purity.

IHC staining of FFPE human bone marrow with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human spleen tissue lysate using recombinant MPO antibody (clone rMPO/6904). Expected molecular weight: 75-90 kDa (pro form).
SDS-PAGE analysis of purified, BSA-free MPO antibody (clone rMPO/6904) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the recombinant MPO antibody should be determined by the researcher.
Description:
Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 150-250 was used as the immunogen for the recombinant MPO antibody.
Limitation:
This recombinant MPO antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P05164