Vimentin Antibody

NSJ Bioreagents
Product Code: NSJ-V9293SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9293SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rVIM/6575
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human U-87 MG cell lysate using Vimentin antibody (clone rVIM/6575). Predicted molecular weight ~53 kDa.
2 / 4
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone rVIM/6575) as confirmation of integrity and purity.

Western blot testing of human U-87 MG cell lysate using Vimentin antibody (clone rVIM/6575). Predicted molecular weight ~53 kDa.
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone rVIM/6575) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Vimentin antibody should be determined by the researcher.
Description:
Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Vimentin protein was used as the immunogen for the Vimentin antibody.
Limitation:
This Vimentin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
P08670