Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-V9284SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9284SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: VIM/6430R
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Feline (cat)
  • Horse
  • Human
  • Mouse
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 10
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE dog kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE cat kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE horse small intestine tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE horse lung tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE cow brain tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
9 / 10
Western blot testing of human U-87 MG cell lysate with recombinant Vimentin antibody. Predicted molecular weight ~53 kDa.
10 / 10
SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6430R) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE dog kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cat kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse small intestine tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse lung tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cow brain tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human U-87 MG cell lysate with recombinant Vimentin antibody. Predicted molecular weight ~53 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6430R) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.
Description:
Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
Limitation:
This recombinant Vimentin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
P08670