PAX5 Antibody

NSJ Bioreagents
Product Code: NSJ-V9267SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9267SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: PAX5/3977R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant PAX5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human tonsil tissue with recombinant PAX5 antibody (clone PAX5/3977R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human tonsil tissue with recombinant PAX5 antibody (clone PAX5/3977R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant PAX5 antibody (clone PAX5/3977R) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil tissue with recombinant PAX5 antibody (clone PAX5/3977R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant PAX5 antibody (clone PAX5/3977R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant PAX5 antibody (clone PAX5/3977R) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant PAX5 antibody should be determined by the researcher.
Description:
The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 235-382 from the human protein was used as the immunogen for the recombinant PAX5 antibody.
Limitation:
This recombinant PAX5 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Uniprot #:
Q02548