WT1 Antibody / Wilms Tumor 1

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NSJ Bioreagents
Product Code: NSJ-V8926SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8926SAF-100UG100ug£534.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: WT1/3477R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant Wilms Tumor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human kidney tissue with recombinant Wilms Tumor 1 antibody (clone WT1/3477R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human ovarian carcinoma tissue with recombinant Wilms Tumor 1 antibody (clone WT1/3477R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Negative control: IHC staining of FFPE human kidney tissue PBS instead of primary antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney tissue with recombinant Wilms Tumor 1 antibody (clone WT1/3477R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant Wilms Tumor 1 antibody (clone WT1/3477R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human kidney tissue PBS instead of primary antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Wilms Tumor 1 antibody should be determined by the researcher.
Description:
The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-100 was used as the immunogen for the recombinant Wilms Tumor 1 antibody.
Limitation:
This recombinant Wilms Tumor 1 antibody is available for research use only.
Localization:
Nucleus and cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P19544