Caldesmon Antibody / CALD1 / CAD (HMW)
| Code | Size | Price |
|---|
| NSJ-V8878SAF-100UG | 100ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rCALD1/820
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot the recombinant Caldesmon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
Application Note:
Optimal dilution of the recombinant Caldesmon antibody should be determined by the researcher.
Description:
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody.
Limitation:
This recombinant Caldesmon antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
Q05682