PD-L1 Antibody / B7-H1 / CD274

Contact us
NSJ Bioreagents
Product Code: NSJ-F55054
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55054-0.1ML0.1ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: AC37
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the PD-L1 antibody at 2-8oC.

Images

1 / 5
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC staining of FFPE human esophageal squamous carcinoma tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
4 / 5
IHC staining of FFPE human NCI-H226 cells (left) and PD-L1 transfected HEK293 cells (right) with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
5 / 5
Western blot testing of PD-L1 transfected human 293 cell lysate (left) and non-transfected 293 cell lysate (right). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
❮❯

IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human esophageal squamous carcinoma tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human NCI-H226 cells (left) and PD-L1 transfected HEK293 cells (right) with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of PD-L1 transfected human 293 cell lysate (left) and non-transfected 293 cell lysate (right). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

Documents

Further Information

Application Details:
Western blot: 1:500-1:1000,Immunohistochemistry (FFPE): 1:100-1:400
Application Note:
Optimal dilution of the PD-L1 antibody should be determined by the researcher.
Description:
As a ligand for the inhibitory receptor PDCD1/PD-1, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response. [UniProt]
Format:
Purified
Formulation:
Antibody in 1X PBS with 0.05% BSA and 0.05% ProClin 300
Limitation:
This PD-L1 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
Q9NZQ7