CD15 Antibody / SSEA-1

NSJ Bioreagents
Product Code: NSJ-V3203SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3203SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

Documents

Further Information

Application Details:
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 5-10ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD15 antibody should be determined by the researcher.
Description:
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Purified human neutrophils were used as the immunogen for the CD15 antibody.
Limitation:
This CD15 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein A affinity
Uniprot #:
P22083