EZH2 Antibody

Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Titration of the EZH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

EZH2, SUZ12, and EED form a complex that methylates nucleosomal histone H3 at Lys27. EZH2 contains a SET domain, a signature motif for all known histone lysine methyltransferases except the H3-K79 methyltransferase DOT1, and is therefore likely to be the catalytic subunit. Consequently, EZH2 is thought to regulate gene expression by controlling chromatin structure. Several lines of evidence suggested a critical role for the EZH2 protein during normal and perturbed development of the hematopoietic and central nervous systems. The EZH2 protein has been shown to associate with the VAV1 protooncoprotein and with the XNP protein, the product of a gene associated with mental retardation. Additionally, due to mapping of EZH2 to the 7q35-q36 chromosomal region associated with myeloid disorders, this protein is suggested to participate in the genetic events triggering myeloid leukemia.

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

This EZH2 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EZH2.

This EZH2 antibody is available for research use only.

Antigen affinity

Q15910